DNA hybridization in thermoresponsive polymer nanoparticles.
نویسندگان
چکیده
We achieve very high hybridization efficiencies by using a new method to immobilize DNA strands on the surface of thermoresponsive polymer nanoparticles. Hybridization efficiencies of about 70 % are obtained between the DNA immobilized in the particles and a complementary strand in solution, even at very low ionic strengths (1 mM). The polymer nanoparticles have a glassy poly(methylmethacrylate) (PMMA) core and a thermoresponsive shell of poly(N-isopropylacrylamide) (PNIPAM) containing positive charges. After a DNA strand labeled with a fluorescence probe is loaded onto the particles at room temperature, the temperature is increased above the volume phase transition temperature of the PNIPAM shell, TVPT approximately 28 degrees C. The collapse of the particle shell immobilizes the DNA while maintaining its availability for hybridization with a complementary strand. Förster resonance energy transfer (FRET) is used to detect the hybridization with a complementary DNA strand labeled with a FRET acceptor probe.
منابع مشابه
Thermoresponsive nanoparticles + plasmonic nanoparticles = photoresponsive heterodimers: facile synthesis and sunlight-induced reversible clustering.
Magnetic nanoparticles functionalized with thermoresponsive polymers become photoresponsive when attached to plasmonic nanoparticles to form heterodimers. Under solar illumination, the plasmonic particles absorb sunlight and induce localized heating, causing collapse of the thermoresponsive polymer chain and clustering of the dimers that facilitate magnetic separation.
متن کاملFluorescence of oligonucleotides adsorbed onto the thermoresponsive poly(isopropyl acrylamide) shell of polymer nanoparticles: Application to bioassays*
The fluorescence of a rhodamine X dye covalently linked to the 5' terminus of a 25-mers thymine oligodeoxynucleotide (dT25-ROX), adsorbed on the shell of thermo responsive core-shell polymer particles, was used to probe the polarity, mobility, and distribution of the oligodeoxynucleotides (ODNs) in the shell. The particles have a glassy core of poly(methyl methacrylate) (PMMA) with a 67-nm radi...
متن کاملThermoresponsive core-shell magnetic nanoparticles for combined modalities of cancer therapy.
Thermoresponsive polymer-coated magnetic nanoparticles loaded with anti-cancer drugs are of considerable interest for novel multi-modal cancer therapies. Such nanoparticles can be used for magnetic drug targeting followed by simultaneous hyperthermia and drug release. Gamma-Fe(2)O(3) iron oxide magnetic nanoparticles (MNP) with average sizes of 14, 19 and 43 nm were synthesized by high temperat...
متن کاملThermoresponsive Interplay of Water Insoluble Poly(2-alkyl-2-oxazoline)s Composition and Supramolecular Host–Guest Interactions
A series of water insoluble poly[(2-ethyl-2-oxazoline)-ran-(2-nonyl-2-oxazoline)] amphiphilic copolymers was synthesized and their solubility properties in the presence of different supramolecular host molecules were investigated. The resulting polymer-cavitand assemblies exhibited a thermoresponsive behavior that could be modulated by variation of the copolymer composition and length. Interest...
متن کاملThe Importance of Excess Poly(N-isopropylacrylamide) for the Aggregation of Poly(N-isopropylacrylamide)-Coated Gold Nanoparticles.
Thermoresponsive materials are generating significant interest on account of the sharp and tunable temperature deswelling transition of the polymer chain. Such materials have shown promise in drug delivery devices, sensing systems, and self-assembly. Incorporation of nanoparticles (NPs), typically through covalent attachment of the polymer chains to the NP surface, can add additional functional...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemphyschem : a European journal of chemical physics and physical chemistry
دوره 11 8 شماره
صفحات -
تاریخ انتشار 2010